Background：notch 1(NOTCH1) Homo sapiens This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types.
| Item | Details |
|---|---|
| Notch1 Rabbit mAb | WR4123 |
| 0.22 Nitrocellulose Membrane | W3N21 |
| Item | Details |
|---|---|
| pre-staining standard protein Marker | (10-180kDa):W2100 |
| Lane Number | Cell Information |
|---|---|
| Lane1 | HL60 – Human promyelocytic leukemia cell |
| Lane2 | HUVEC – Human umbilical vein endothelial cell |
| Lane3 | Human CAF – Human cancer-associated fibroblast |
| Lane4 | HCT116 – Human colorectal carcinoma |
| Lane5 | A549 – Human lung carcinoma |
| Lane6 | Jurkat – Human T lymphocyte leukemia |
| Lane7 | HT22 – Mouse hippocampal neuronal |
| Lane8 | Thp-1 – Human monocytic leukemia |
| Lane9 | HK-2 – Human proximal tubular epithelial |
| Lane10 | PC-12 – Rat adrenal pheochromocytoma |
| Lane11 | 3T3-L1 – Mouse embryonic fibroblast cells |
| Lane12 | PC-3 – Human prostate adenocarcinoma |
| Lane13 | U-14 – Mouse cervical carcinoma |
| Lane14 | 293F – HEK293 derivative, adapted for suspension culture |
| Item | Details |
|---|---|
| SDS-PAGE separation | Various whole cell lysates were separated by 4-20% SDS-PAGE |
| Primary antibody conditions | Used at 4°C, over night with a 1:2500 dilution |
| Detection antibody | Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446) |
| Item | Band Size |
|---|---|
| Predicted band size | 273 kDa |
| Observed band size | 120 kDa |
Elevate your Notch signaling pathway and developmental biology research with the Notch1 Rabbit Monoclonal Antibody (WR4123)—a Google-optimized, rigorously validated tool for specific Notch1 detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, A549, etc.), this antibody delivers clear, consistent banding (273 kDa predicted / 120 kDa observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a robust Dylight 800-conjugated Goat anti-Rabbit detection system.
Ideal for studying Notch1’s roles as a Type I transmembrane protein in the evolutionarily conserved Notch signaling pathway, its function in cell-cell interaction regulation, proteolytic processing mechanism, or its critical role in the development of diverse cell and tissue types—critical for developmental biology, signal transduction, and cell fate determination research—this trusted Notch1 Rabbit Monoclonal Antibody boosts assay reproducibility for Notch pathway studies, developmental genetics, and intercellular signaling research. Order WR4123 today to advance your Notch1-focused experiments with a SEO-verified, high-performance research tool tailored for Notch signaling and developmental biology studies.
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