Background：The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
| Item | Details |
|---|---|
| LC3B Rabbit mAb | WR4126 |
| 0.22 Nitrocellulose Membrane | W3N21 |
| Item | Details |
|---|---|
| pre-staining standard protein Marker | (10-180kDa):W2100 |
| Lane Number | Cell Information |
|---|---|
| Lane1 | HL60 – Human promyelocytic leukemia cell |
| Lane2 | HUVEC – Human umbilical vein endothelial cell |
| Lane3 | Human CAF – Human cancer-associated fibroblast |
| Lane4 | HCT116 – Human colorectal carcinoma |
| Lane5 | A549 – Human lung carcinoma |
| Lane6 | Jurkat – Human T lymphocyte leukemia |
| Lane7 | HT22 – Mouse hippocampal neuronal |
| Lane8 | Thp-1 – Human monocytic leukemia |
| Lane9 | HK-2 – Human proximal tubular epithelial |
| Lane10 | PC-12 – Rat adrenal pheochromocytoma |
| Lane11 | 3T3-L1 – Mouse embryonic fibroblast cells |
| Lane12 | PC-3 – Human prostate adenocarcinoma |
| Lane13 | U-14 – Mouse cervical carcinoma |
| Lane14 | 293F – HEK293 derivative, adapted for suspension culture |
| Item | Details |
|---|---|
| SDS-PAGE separation | Various whole cell lysates were separated by 4-20% SDS-PAGE |
| Primary antibody conditions | Used at 4°C, over night with a 1:5000 dilution |
| Detection antibody | Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446) |
| Item | Band Size |
|---|---|
| Predicted band size | 14、16 kDa |
| Observed band size | 14、16 kDa |
Elevate your autophagy and neuronal microtubule research with the LC3B Rabbit Monoclonal Antibody (WR4126)—a Google-optimized, rigorously validated tool for specific LC3B detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, HT22, etc.), this antibody delivers perfect band alignment (14、16 kDa predicted/observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:5000 dilution) and a robust Dylight 800-conjugated Goat anti-Rabbit detection system.
Ideal for studying LC3B’s roles as a subunit of neuronal microtubule-associated MAP1A/MAP1B proteins, its critical function in microtubule assembly and neurogenesis, or its well-established involvement in autophagy (the bulk degradation of cytoplasmic components)—critical for autophagy biology, neuroscience, and cellular homeostasis research—this trusted LC3B Rabbit Monoclonal Antibody boosts assay reproducibility for autophagic flux studies, neuronal development research, and microtubule dynamics analysis. Order WR4126 today to advance your LC3B-focused experiments with a SEO-verified, high-performance research tool tailored for autophagy and neuronal microtubule studies.
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