Background：VEGF receptor1 related tyrosine kinase 1(FLT1) Homo sapiens This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes.
| Product Identification | Details | Catalog No. |
|---|---|---|
| Antibody Name | VEGF receptor1 Rabbit mAb | WR4009 |
| Reagent Type | Reagent Name | Catalog No./Specification |
|---|---|---|
| Transfer Membrane | 0.22 Nitrocellulose Membrane | W3N21 |
| Protein Marker | pre-staining standard protein Marker(10-250kDa) | W2101 |
| Secondary Antibody | Dylight 800-conjugated Goat anti-Rabbit antibody | Cat:WS0446 |
| Lane Number | Cell Line | Cell Type Description |
|---|---|---|
| Lane1 | HL60 | Human promyelocytic leukemia cell |
| Lane2 | HUVEC | Human umbilical vein endothelial cell |
| Lane3 | Human CAF | Human cancer-associated fibroblast |
| Lane4 | HCT116 | Human colorectal carcinoma |
| Lane5 | A549 | Human lung carcinoma |
| Lane6 | Jurkat | Human T lymphocyte leukemia |
| Lane7 | HT22 | Mouse hippocampal neuronal |
| Lane8 | Thp-1 | Human monocytic leukemia |
| Lane9 | HK-2 | Human proximal tubular epithelial |
| Lane10 | PC-12 | Rat adrenal pheochromocytoma |
| Lane11 | 3T3-L1 | Mouse embryonic fibroblast cells |
| Lane12 | PC-3 | Human prostate adenocarcinoma |
| Lane13 | U-14 | Mouse cervical carcinoma |
| Lane14 | 293F | HEK293 derivative, adapted for suspension culture |
| Experimental Step | Operation Details |
|---|---|
| Sample Separation | Various whole cell lysates were separated by 4-20% SDS-PAGE |
| Primary Antibody Incubation | Primary antibody was used at 4°C, over night with a 1:2500 dilution |
| Secondary Antibody Detection | Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446) was used to detect the antibody |
| Band Type | Size |
|---|---|
| Predicted band | 151 kDa |
| Observed band | 180 kDa |
Looking to advance your research on human VEGF Receptor 1 (FLT1) efficiently? This VEGF Receptor 1 Rabbit Monoclonal Antibody (Catalog No. WR4009) is your reliable choice! Rigorously validated, it accurately recognizes the target protein—across a wide range of human cell lines (including HL60, HUVEC, HCT116) as well as mouse/rat cells, it exhibits a clear observed band at 180 kDa (matching the 151 kDa predicted size) when detected via 4-20% SDS-PAGE. It is perfectly suited for mechanistic studies of angiogenesis and vasculogenesis.
What’s more, it can be seamlessly paired with our complementary reagents: 0.22μm Nitrocellulose Transfer Membrane (W3N21), pre-staining Standard Protein Marker (10-250kDa, W2101), and Dylight 800-conjugated Goat anti-Rabbit Secondary Antibody (WS0446), providing a one-stop solution for your experimental reagent needs and significantly boosting research efficiency.
Search for “VEGFR1 Rabbit mAb”, “human FLT1 detection antibody”, “HL60 cell VEGFR1 antibody”, or “angiogenesis research VEGFR1 monoclonal antibody” to get this high-specificity, highly versatile experimental tool today—and accelerate your research breakthroughs!
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