PI3 Kinase P85α Rabbit Monoclonal antibody

PI3 Kinase P85α Rabbit Monoclonal antibody

Background:Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

 

Category Item Description
Antibody & Materials Primary Antibody PI3K-kinase p85a Rabbit mAb[PT0082R]:WR4026
Antibody & Materials Nitrocellulose Membrane 0.22 Nitrocellulose Membrane:W3N21
Antibody & Materials Protein Marker pre-staining standard protein Marker(10-180kDa):W2100
Lane Cell Type
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Step Description
Electrophoresis Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary Antibody Incubation The primary antibody was used at 4°C, over night with a 1:5000 dilution
Secondary Antibody Detection The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody
Type Size
Predicted band size 84 kDa
Observed band size 84 kDa

Elevate your PI3K signaling and cancer research with the highly specific PI3 Kinase P85α Rabbit Monoclonal Antibody. Validated across diverse cell lines and optimized for Western Blot, it’s the go-to tool for investigating PI3K-mediated cell growth, insulin resistance, and oncogenic pathways. Accelerate discoveries in oncology and metabolic biology with this top-tier antibody today!

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