METTL3 Rabbit Monoclonal Antibody

METTL3 Rabbit Monoclonal Antibody

Background:This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Item Details
METTL3 Rabbit mAb WR4087
0.22 Nitrocellulose Membrane W3N21
Item Details
pre-staining standard protein Marker (10-180kDa):W2100
Lane Number Cell Information
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Item Details
SDS-PAGE separation Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary antibody conditions Used at 4°C, over night with a 1:2500 dilution
Detection antibody Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446)
Item Band Size
Predicted band size 64 kDa
Observed band size 70 kDa

Elevate your N6-adenosine methylation research with the METTL3 Rabbit Monoclonal Antibody (WR4087)—a Google-optimized, rigorously validated tool for specific METTL3 detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, HT22, etc.), this antibody delivers clear banding (64 kDa predicted / 70 kDa observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a robust Dylight 800-conjugated detection system.

Ideal for studies of posttranscriptional mRNA modification (N6-methyladenosine) mediated by MT-A’s 70 kDa subunit, this trusted METTL3 Rabbit Monoclonal Antibody boosts assay reproducibility. Order WR4087 today to advance your METTL3-focused experiments with a SEO-verified, high-performance research tool.

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