alpha-Tubulin Rabbit Monoclonal antibody

alpha-Tubulin Rabbit Monoclonal antibody

Background:function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.

Item Details
TUBA1B Rabbit mAb WR4105
0.22 Nitrocellulose Membrane W3N21
Item Details
pre-staining standard protein Marker (10-180kDa):W2100
Lane Number Cell Information
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Item Details
SDS-PAGE separation Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary antibody conditions Used at 4°C, over night with a 1:2500 dilution
Detection antibody Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446)
Item Band Size
Predicted band size 50 kDa
Observed band size 50 kDa

Elevate your cell cytoskeleton and microtubule research with the alpha-Tubulin Rabbit Monoclonal Antibody (WR4105, TUBA1B)—a Google-optimized, rigorously validated tool for specific alpha-tubulin detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, 3T3-L1, etc.), this antibody delivers perfect band alignment (50 kDa predicted/observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a reliable Dylight 800-conjugated Goat anti-Rabbit detection system.

Ideal for studying microtubule structure/function (as a major tubulin constituent), GTP-binding dynamics, the tubulin tyrosination/detyrosination cycle, or alpha-beta tubulin dimer interactions—critical for cell division, cytoskeletal remodeling, and intracellular trafficking—this trusted alpha-Tubulin Rabbit Monoclonal Antibody boosts assay reproducibility for cell biology, cancer, and developmental research. Order WR4105 today to advance your tubulin-focused experiments with a SEO-verified, high-performance research tool tailored for cytoskeleton and microtubule dynamics studies.

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