Price range: $268.00 through $328.00
Background:This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
| Target | Myt 1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 55kD |
| MW(Observed) | 70kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | IHC 1:200-1:500; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Source | |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | PT1612R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Sequence | |
| Specificity | Endogenous |
| Gene Name | PKMYT1 |
| Protein Name | Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase |
| Other Name | PKMYT1; / MYT1; / Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; / Myt1 kinase |
| Species | Human |
| Gene ID-1 | 9088 |
| Uniprot | Q99640, |
| Species.1 | Mouse |
| Gene ID-2 | |
| Uniprot.1 | Q9ESG9 |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012], |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein . Golgi apparatus membrane; Peripheral membrane protein . |
| Tissue Expression | Brain,Epithelium,PCR rescued clones, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle |
| Research Areas | >>Cell cycle; / >>Oocyte meiosis; / >>Progesterone-mediated oocyte maturation |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.,enzyme regulation:Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation.,Function:Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the cdc2 kinase specifically when cdc2 is complexed to cyclins. Mediates phosphorylation of cdc2 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of cdc2 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.,PTM:Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.,sequence Caution:Chimeric cDNA.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1., |
| RRID |
