Price range: $268.00 through $328.00
Background:This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | FoxO1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 70kD |
| MW(Observed) | 75kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:400-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0213R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | FOXO1 |
| Protein Name | Forkhead box protein O1 |
| Other Name | FOXO1; / FKHR; / FOXO1A; / Forkhead box protein O1; / Forkhead box protein O1A; / Forkhead in rhabdomyosarcoma |
| Species | Human |
| Gene ID | 2308 |
| Uniprot | Q12778, |
| Species.1 | Mouse |
| Gene ID.1 | 56458 |
| Uniprot.1 | Q9R1E0, |
| Species.2 | Rat |
| Gene ID.2 | 84482 |
| Uniprot.2 | G3V7R4 |
| Cellular Localization | Nucleus |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cellular senescence;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Prostate cancer;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Cellular senescence; / >>Insulin signaling pathway; / >>Thyroid hormone signaling pathway; / >>Glucagon signaling pathway; / >>Insulin resistance; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Alcoholic liver disease; / >>Shigellosis; / >>Human papillomavirus infection; / >>Pathways in cancer; / >>Transcriptional misregulation in cancer; / >>Prostate cancer |
| Function | Disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2; 13)(q35; q14) with PAX3; translocation t(1; 13)(p36; q14) with PAX7. The resulting protein is a transcriptional activator.,Function:Transcription factor.,PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:Shuttles between cytoplasm and nucleus.,subunit:Interacts with LRPPRC.,tissue specificity:Ubiquitous., |
