Name: U-Blot® SPTBN1 Rabbit mAb
SKU: WR4432
Price: 268 USD
Availability: InStock

U-Blot® SPTBN1 Rabbit mAb

Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4432 Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4432-50

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Product details

Background:Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	SPTBN1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	275kD
MW(Observed)	275kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0426R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	SPTBN1
Protein Name	Spectrin beta chain non-erythrocytic 1
Other Name	SPTBN1; / SPTB2; / Spectrin beta chain; / non-erythrocytic 1; / Beta-II spectrin; / Fodrin beta chain; / Spectrin, non-erythroid beta chain 1
Species	Human
Gene ID	4040
Uniprot	Q01082,
Species.1	Mouse
Gene ID.1	20742
Uniprot.1	Q62261
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm, Membrane
Tissue Expression	Widely coexpressed with LRP5 during embryogenesis and in adult tissues.
Signaling_pathway	Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>mTOR signaling pathway; / >>Wnt signaling pathway; / >>Parathyroid hormone synthesis, secretion and action; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Pathways in cancer; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer
Function	Function:Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.,PTM:Isoform 2 is phosphorylated on Ser-8 and Ser-10.,similarity:Belongs to the spectrin family.,similarity:Contains 1 PH domain.,similarity:Contains 17 spectrin repeats.,similarity:Contains 2 CH (calponin-homology) domains.,subcellular location:Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes.,subunit:Like erythrocyte spectrin, the spectrin-like proteins are capable to form dimers which can further associate to tetramers. The short form cannot bind to the axonal protein fodaxin. Interacts with ANK2.,tissue specificity:Isoform 2 is present in brain, lung and kidney (at protein level).,