Product details

Background:
P40 (△ Np63) is one of the subtypes of p63 protein and the main p63 isoform in basal cells / progenitor cells. It is expressed in urothelial cells and squamous cells, myoepithelial cells of breast, sweat gland and salivary gland, and basal cells of prostate. P40 has a high sensitivity similar to p63 in lung squamous cell carcinoma, but it is rarely expressed in lung adenocarcinoma, so P40 is used in the differential diagnosis of lung squamous cell carcinoma and lung adenocarcinoma.

Specifications

Size	50μL/100μL
Target	p40/p63
Reactivity	Human, Mouse
Applications	IHC, WB, IF, ELISA
MW(Calculated)	77kD
MW(Observed)	77kD,69kD
Host Species	Mouse
Isotype	IgG2b,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RMM020
Immunogen	Synthesized peptide derived from human p63 AA range: 1-100
Specificity	The antibody can specifically recognize human P40(ΔNp63) protein, and TAp63 dose not respond.
Gene Name	TP63 KET P63 P73H P73L TP73L
Protein Name	Tumor protein 63 (p63) (Chronic ulcerative stomatitis protein) (CUSP) (Keratinocyte transcription factor KET) (Transformation-related protein 63) (TP63) (Tumor protein p73-like) (p73L) (p40) (p51)
Other Name	AIS; / Amplified in squamous cell carcinoma; / B; / p51A; / B; / p51B; / Chronic ulceRative stomatitis protein; / CUSP; / DN p63 alpha 1; / DNp63; / EEC3; / id:ibd3516; / KeRatinocyte transcription factor; / KeRatinocyte transcription factor KET; / KET; / LMS; / MGC115972; / MGC192897; / NBP; / OFC8; / OTTHUMP00000209732; / OTTHUMP00000209733; / OTTHUMP00000209734; / OTTHUMP00000209735; / OTTHUMP00000209737; / OTTHUMP00000209738; / OTTHUMP00000209739; / OTTHUMP00000209740; / OTTHUMP00000209741; / OTTHUMP00000209742; / OTTHUMP00000209743; / OTTHUMP00000209744; / p40; / p51; / P51/P63; / p53-related protein p63; / p53CP; / p63; / P63_HUMAN; / p73H; / p73L; / RHS; / SHFM4; / TAp63alpha; / TP53CP; / TP53L; / TP63; / TP73L; / Transformation related protein 63; / Transformation-related protein 63; / Trp53rp1; / Trp63; / Tumor protein 63; / Tumor protein p53-competing protein; / Tumor protein p53-like; / Tumor protein p63; / Tumor protein p63 deltaN isoform delta; / Tumor protein p73; / Tumor protein p73-like
Species	Human
Gene ID	8626
Uniprot	Q9H3D4
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nuclear
Tissue Expression	Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
Signaling_pathway	Human Diseases >> Cancer: overview >> MicroRNAs in cancer
Research Areas	>>MicroRNAs in cancer
Function	cofactor:Binds 1 zinc ion per subunit.,Disease:Defects in TP63 are a cause of cervical, colon, head and neck, lung and ovarian cancers.,Disease:Defects in TP63 are a cause of ectodermal dysplasia Rapp-Hodgkin type (EDRH) [MIM:129400]; also called Rapp-Hodgkin syndrome or anhidrotic ectodermal dysplasia with cleft lip/palate. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDRH is characterized by the combination of anhidrotic ectodermal dysplasia, cleft lip, and cleft palate. The clinical syndrome is comprised of a characteristic facies (narrow nose and small mouth), wiry, slow-growing, and uncombable hair, sparse eyelashes and eyebrows, obstructed lacrimal puncta/epiphora, bilateral stenosis of external auditory canals, microsomia, hypodontia, cone-shaped incisors, enamel hypoplasia, dystrophic nails, and cleft lip/cleft palate.,Disease:Defects in TP63 are the cause of acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome) [MIM:103285]; a form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. ADULT syndrome involves ectrodactyly, syndactyly, finger- and toenail dysplasia, hypoplastic breasts and nipples, intensive freckling, lacrimal duct atresia, frontal alopecia, primary hypodontia, and loss of permanent teeth. ADULT differs significantly from EEC3 syndrome by the absence of facial clefting.,Disease:Defects in TP63 are the cause of ankyloblepharon-ectodermal defects-cleft lip/palate (AEC) [MIM:106260]. AEC is an autosomal dominant condition characterized by congenital ectodermal dysplasia with coarse, wiry, sparse hair, dystrophic nails, slight hypohidrosis, scalp infections, ankyloblepharon filiforme adnatum, maxillary hypoplasia, hypodontia and cleft lip/palate.,Disease:Defects in TP63 are the cause of ectrodactyly-ectodermal dysplasia-cleft lip/palate syndrome type 3 (EEC3) [MIM:604292]. EEC3 is an autosomal dominant syndrome characterized by ectrodactyly of hands and feet, ectodermal dysplasia and facial clefting.,Disease:Defects in TP63 are the cause of limb-mammary syndrome (LMS) [MIM:603543]. LMS is characterized by ectrodactyly, cleft palate and mammary-gland abnormalities.,Disease:Defects in TP63 are the cause of non-syndromic orofacial cleft type 8 (OFC8) [MIM:129400]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.,Disease:Defects in TP63 are the cause of split-hand/foot malformation 4 (SHFM4) [MIM:605289]. Split-hand/split-foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. There is restricted overlap between the mutational spectra of EEC3 and SHFM4.,Domain:The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA-type isoforms.,Function:Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of TP53/p53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.,PTM:May be sumoylated.,similarity:Belongs to the p53 family.,similarity:Contains 1 SAM (sterile alpha motif) domain.,subunit:Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity.,tissue specificity:Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.,

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p40 [ABT020] mouse mAb

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p40 [ABT020] mouse mAb

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