Product details

Background:
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Target	Aurora A
Reactivity	Human, Rat, Monkey
Applications	WB, IF, FC, ELISA
MW(Calculated)	46kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	Affinity purification
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	9B12
Immunogen	Purified recombinant fragment of human Aurora A expressed in E. Coli.
Sequence
Specificity	Aurora A Monoclonal Antibody detects endogenous levels of Aurora A protein.
Gene Name	AURKA
Protein Name	Serine/threonine-protein kinase 6
Other Name	AURKA; / AIK; / AIRK1; / ARK1; / AURA; / AYK1; / BTAK; / IAK1; / STK15; / STK6; / Aurora kinase A; / Aurora 2; / Aurora/IPL1-related kinase 1; / ARK-1; / Aurora-related kinase 1; / hARK1; / Breast tumor-amplified kinase; / Serine/threonine-protein kinase 15; / Serine/threonin
Species	Human
Gene ID-1	6790
Uniprot	O14965,
Species.1	Mouse
Gene ID-2
Uniprot.1	P97477,
Species.2	Rat
Gene ID-3
Uniprot.2	P59241
Organism-4
Gene ID-4
SwissProt-4
Background	The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
Cellular Localization	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cell projection, neuron projection . Detected at the neurite hillock in developing neurons (By similarity). Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase (PubMed:9606188, PubMed:17229885, PubMed:21225229). Colocalized with SIRT2 at centrosome (PubMed:22014574). Moves to the midbody during both telophase and cytokinesis (PubMed:17726514). Associates with both the pericentriolar material (PCM) and centrioles (PubMed:22014574). The localization to the spindle poles is regulated by AAAS (PubMed:26246606). .
Tissue Expression	Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Signaling_pathway
Research Areas	>>Oocyte meiosis; / >>Progesterone-mediated oocyte maturation
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Caution:Although authors have considered STK6 and STK15 as two different proteins, it is clear that they are the same protein.,Disease:Defects in AURKA are responsible for numerical centrosome aberrations including aneuploidy.,Function:May play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. May be involved in microtubule formation and/or stabilization. Phosphorylates ARHGEF2 and BORA.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.,subunit:Interacts with TACC1 and CPEB1. Interacts with its substrates BORA and ARHGEF2.,tissue specificity:Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly after.,
RRID

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ARK-1 Monoclonal Antibody

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ARK-1 Monoclonal Antibody

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