Product details

Background:
This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009],

Specifications

Size	50μL/100μL
Target	Flt-1
Reactivity	Human
Applications	WB, ELISA
MW(Calculated)	151kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	3B12
Immunogen	Purified recombinant extracellular fragment of human Flt-1 fused with hIgGFc tag expressed in HEK293 cells.
Specificity	Flt-1 Monoclonal Antibody detects endogenous levels of Flt-1 protein.
Gene Name	FLT1
Protein Name	Vascular endothelial growth factor receptor 1
Other Name	FLT1; / FLT; / FRT; / VEGFR1; / Vascular endothelial growth factor receptor 1; / VEGFR-1; / Fms-like tyrosine kinase 1; / FLT-1; / Tyrosine-protein kinase FRT; / Tyrosine-protein kinase receptor FLT; / FLT; / Vascular permeability factor receptor
Species	Human
Gene ID	2321
Uniprot	P17948,
Species.1	Mouse
Gene ID.1	14254
Uniprot.1	P35969
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	[Isoform 1]: Cell membrane; Single-pass type I membrane protein. Endosome. Autophosphorylation promotes ubiquitination and endocytosis.; [Isoform 2]: Secreted .; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Cytoplasm .; [Isoform 6]: Cytoplasm .; [Isoform 7]: Cytoplasm .
Tissue Expression	Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC).
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Immune disease >> Rheumatoid arthritis;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>MAPK signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>Calcium signaling pathway; / >>HIF-1 signaling pathway; / >>PI3K-Akt signaling pathway; / >>Focal adhesion; / >>Transcriptional misregulation in cancer; / >>Rheumatoid arthritis
Function	Alternative products:Additional isoforms seem to exist,Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Function:Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 7 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Interacts in vitro with various phosphotyrosine-binding proteins, including PLC-gammas, PTPN11, GRB2, CRK and NCK1.,tissue specificity:Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.,

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Flt-1/VEGFR1 Monoclonal Antibody

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Flt-1/VEGFR1 Monoclonal Antibody

Product details

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