Product details

Background:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	HDAC4
Reactivity	Human
Applications	WB, IP
MW(Calculated)
MW(Observed)	140kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	3C9
Immunogen	Purified recombinant human HDAC4 protein fragments expressed in E.coli
Specificity	This antibody detects endogenous levels of HDAC4 and does not cross-react with related proteins
Gene Name	hdac4
Protein Name
Other Name	EC 3.5.1.98; / HA6116; / HD 4; / HD4; / HDAC 4; / HDAC A; / HDAC4; / HDAC4_HUMAN; / HDACA; / Histone Deacetylase 4; / Histone Deacetylase A; / KIAA0288.
Species	Human
Gene ID	9759
Uniprot	P56524,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q6NZM9
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation. Interaction with SIK3 leads to HDAC4 retention in the cytoplasm (By similarity). .
Tissue Expression	Ubiquitous.
Signaling_pathway	Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway
Research Areas	>>Apelin signaling pathway; / >>Neutrophil extracellular trap formation; / >>Alcoholism; / >>Viral carcinogenesis; / >>MicroRNAs in cancer
Function	Catalytic activity:Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.,Domain:The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.,Function:Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.,PTM:Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.,PTM:Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.,similarity:Belongs to the histone deacetylase family. Type 2 subfamily.,subcellular location:Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.,subunit:Interacts with HDAC7 (By similarity). Homodimer. Homodimerization via its N-terminal domain. Interacts with MEF2C, AHRR, and NR2C1. Interacts with a 14-3-3 chaperone protein in a phosphorylation dependent manner. Interacts with BTBD14B (By similarity). Interacts with KDM5B.,tissue specificity:Ubiquitous.,

Please login to post questions

HDAC4 [N-term] mouse mAb

Product details

Specifications

About Us

Support

Order

Menu

HDAC4 [N-term] mouse mAb

Product details

Specifications

Related products

U-Blot® Cleaved PARP1 Rabbit mAb

U-Blot® NLRP3 Rabbit mAb

U-Blot® Cyclin B1 (Phospho Ser126) Rabbit mAb

U-Blot® α smooth muscle actin Rabbit mAb

U-Blot™ SSPE buffer, pH 7.4

U-Blot® FOXO3A (Phospho Ser253) Rabbit mAb

Customer Reviews

Menu