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U-Blot® SMUF2 Rabbit mAb
Ucallm_WR6036-50
Price range: $268.00 through $328.00
Specifications
| Size | 50μL/100μL |
| Target | SMUF2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 86kD |
| MW(Observed) | 86kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM2020R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | SMURF2 |
| Protein Name | SMUF2 |
| Other Name | |
| Species | Human |
| Gene ID | 64750 |
| Uniprot | Q9HAU4, |
| Species.1 | Mouse |
| Gene ID.1 | 66313 |
| Uniprot.1 | A2A5Z6 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . Cell membrane . Membrane raft . Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts. |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Environmental Information Processing >> Signal transduction >> Hedgehog signaling pathway;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis |
| Research Areas | >>Ubiquitin mediated proteolysis; / >>Endocytosis; / >>Hedgehog signaling pathway; / >>TGF-beta signaling pathway |
| Function | Domain:The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.,Domain:The second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).,Function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 C2 domain.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 3 WW domains.,subcellular location:Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.,subunit:Interacts (via WW domains) with SMAD1. Interacts (via WW domains) with SMAD2 (via PY-motif). Interacts (via WW domains) with SMAD3 (via PY-motif). Interacts with SMAD6. Interacts with SMAD7 (via PY-motif). Does not interact with SMAD4; SMAD4 lacks a PY-motif. Interacts with SCYE1.,tissue specificity:Widely expressed., |
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