Product details

Background：
cytochrome P450 family 2 subfamily C member 19 (CYP2C19) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol , steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics , including the anticonvulsive drug mephenytoin , omeprazole , diazepam and some barbiturates. Polymorphism within this gene is associated with variable ability to metabolize mephenytoin , known as the poor metabolizer and extensive metabolizer phenotypes. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq , Jul 2008] ,

Specifications

Size	50μL/100μL
Target	CYP2C19
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	56kD
MW(Observed)	56kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1954R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	CYP2C19
Protein Name	Cytochrome P450 2C19
Other Name	CYP2C19; / Cytochrome P450 2C19; / R; / -limonene 6-monooxygenase; / S; / -limonene 6-monooxygenase; / S; / -limonene 7-monooxygenase; / CYPIIC17; / CYPIIC19; / Cytochrome P450-11A; / Cytochrome P450-254C; / Mephenytoin 4-hydroxylase
Species	Human
Gene ID	1557
Uniprot	P33261
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue Expression	Liver,
Signaling_pathway
Research Areas	>>Arachidonic acid metabolism; / >>Linoleic acid metabolism; / >>Drug metabolism - cytochrome P450; / >>Metabolic pathways; / >>Serotonergic synapse; / >>Chemical carcinogenesis - DNA adducts
Function	Catalytic activity:(+)-(R)-limonene + NADPH + O(2) = (+)-trans-carveol + NADP(+) + H(2)O.,Catalytic activity:(-)-(S)-limonene + NADPH + O(2) = (-)-perillyl alcohol + NADP(+) + H(2)O.,Catalytic activity:(-)-(S)-limonene + NADPH + O(2) = (-)-trans-carveol + NADP(+) + H(2)O.,Caution:P450-254C was originally listed as a separate gene (CYP2C17). Resequencing demonstrated that it is not a separate gene, but a chimera. The 5'-portion corresponds to a partial 2C18 clone, and the 3'-portion corresponds to a partial 2C19 clone.,cofactor:Heme group.,Function:Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, citalopram and imipramine.,induction:P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.,online information:CYP2C19 alleles,polymorphism:Genetic variation in CYP2C19 is responsible for poor drug metabolism [MIM:609535]. Individuals can be characterized as either extensive metabolizers (EM) or poor metabolizers (PM). The PM phenotype is inherited in an autosomal recessive manner, with the EM phenotype comprising both homozygous dominant and heteroyzgote genotypes. There are marked interracial differences in the frequency of this polymorphism. Poor metabolizers represent 2-5% of Caucasians, 13-23% of Asian populations, and as many as 38-79% of individuals of some of the islands of Polynesia and Micronesia. Different alleles of CYP2C19 are known: CYP2C191A CYP2C191B, CYP2C191C, CYP2C192A (CYP2C19m1 or CYP2C19m1A), CYP2C192B (CYP2C19m1B), CYP2C192C (CYP2C1921), CYP2C193A (CYP2C19m2), CYP2C193B (CYP2C1920), CYP2C194 (CYP2C19m3), CYP2C195A (CYP2C19m4), CYP2C195B, CYP2C196, CYP2C197, CYP2C198, CYP2C199, CYP2C1910, CYP2C1911 CYP2C1912, CYP2C1913, CYP2C1914 CYP2C1915, CYP2C1916, CYP2C1918, CYP2C1919. Defective CYP2C192 and CYP2C193 alleles are characterized by a splice mutation and a stop codon, respectively, and account for most of the PM alleles. The sequence shown is that of allele CYP2C19*1B.,similarity:Belongs to the cytochrome P450 family.,

Please login to post questions

U-Blot® CYP2C19 Rabbit mAb

Product details

Specifications

About Us

Support

Order

Menu

U-Blot® CYP2C19 Rabbit mAb

Product details

Specifications

Related products

U-Blot® NLRP3 Rabbit mAb

U-Blot® Smad2 (Phospho Ser250) Rabbit mAb

U-Blot® Ezrin Rabbit mAb

U-Blot® MMP7 Rabbit mAb

U-Blot® Cyclin B1 (Phospho Ser126) Rabbit mAb

U-Blot® SQSTM1/p62 Rabbit mAb

Customer Reviews

Menu