$999.00
Background:CARM1 (Coactivator-associated arginine methyltransferase 1 or CARM 1) is a protein arginine N-methyltransferase that catalyzes the mono- and asymmetric dimethylation of arginine residues in its substrate proteins. It serves as a coactivator of transcription and also plays a role in nuclear receptors. CARM1 methylates histone H3 at Arg2, Arg17 and Arg26; it also methylates a number of non-histone substrates involved in the regulation and mechanism of gene expression.
| Target | -- |
| Reactivity | Human |
| Applications | ChIP; ChIP-Seq; WB |
| MW(Calculated) | 145 kDa |
| Host Species | Rabbit |
| Isotype | IgG |
| Recommended Dilution Ratio | Applications Validated by Active Motif: ChIP: 10 µl per ChIP ChIP-Seq: 10 µl each WB*: 1:500 - 1:1,000 dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services. The complete data set is available in the UCSC Genome Browser by clicking here . *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. |
| Formulation | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Purification | Affinity Purified |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Polyclonal |
| Clone Number | -- |
| Immunogen | This BRD8 / SMAP2 antibody was raised against a peptide corresponding to amino acids within the N-terminal region of human BRD8. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | SMAP, p120 |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
