SKU: WR4874 Categories: ,

U-Blot® DGCR8 Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR4874-50
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Product details

Background:This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010],

Specifications

Size50μL/100μL
TargetDGCR8
ReactivityHuman, Mouse, Rat
ApplicationsWB, IF, IP, ELISA
MW(Calculated)86kD
MW(Observed)120kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200;
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1044R
ImmunogenSynthesized peptide derived from human N-ternal DGCR8.
SpecificityEndogenous
Gene NameDGCR8 C22orf12 DGCRK6 LP4941
Protein NameDGCR8
Other NameMicroprocessor complex subunit DGCR8; / DiGeorge syndrome critical region 8
SpeciesHuman
Gene ID54487
UniprotQ8WYQ5,
Species.1Mouse
Gene ID.194223
Uniprot.1Q9EQM6
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus . Nucleus, nucleolus . Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies (PubMed:17159994).
Tissue ExpressionUbiquitously expressed.
Signaling_pathway
Research Areas
FunctionDisease:May play a part in the etiology of the velocardiofacial/DiGeorge syndrome (VCFS/DGS), a developmental disorder characterized by structural and functional palate anomalies, conotruncal cardiac malformations, immunodeficiency, hypocalcemia, and typical facial anomalies. Most cases result from a deletion of chromosome 22q11.2 (the DiGeorge syndrome chromosome region, or DGCR).,similarity:Contains 1 WW domain.,similarity:Contains 2 DRBM (double-stranded RNA-binding) domains.,tissue specificity:Ubiquitously expressed.,
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