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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/Smad3 (phospho Ser204) Polyclonal Antibody
SKU: WP9396 Categories: ,

Smad3 (phospho Ser204) Polyclonal Antibody

Ucallm_WP9396-50

Price range: $288.00 through $368.00

SKU: WP9396-100
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Product details

Background:

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],

Specifications

TargetSmad3
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)48kD
Host SpeciesRabbit
IsotypeIgG
Conjugate
ModificationPhospho
Recommended Dilution RatioWB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
CompositionLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification ProcessThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage Condition-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser204. AA range:170-219
Sequence
SpecificityPhospho-Smad3 (S204) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S204.
Gene NameSMAD3
Protein NameMothers against decapentaplegic homolog 3
AliasSMAD3;MADH3;Mothers against decapentaplegic homolog 3;MAD homolog 3;Mad3;Mothers against DPP homolog 3;hMAD-3;JV15-2;SMAD family member 3;SMAD 3;Smad3;hSMAD3
Organism-1Human
Gene ID-14088
SwissProt-1P84022
Organism-2Mouse
Gene ID-217127
SwissProt-2Q8BUN5
Organism-3Rat
Gene ID-325631
SwissProt-3P84025
Organism-4
Gene ID-4
SwissProt-4
Cellular LocalizationCytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity). .
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