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Background:

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014],

Specifications

Size	50μL/100μL
Target	MKK7 Phospho Ser271/Thr275
Reactivity	Human, Mouse, Rat
Applications	WB, ELISA, IHC
MW(Calculated)
MW(Observed)	47kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	phosphate
Recommended Dilution Ratio	WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthesized phosho peptide around human MKK7 (Ser271 and Thr275)
Specificity	This antibody detects endogenous levels of MKK7 only when phosphorylated at Ser271 or Thr275,and dually phosphorylated at two sites.
Gene Name	MAP2K7 JNKK2 MEK7 MKK7 PRKMK7 SKK4
Protein Name	MKK7 (Ser271/Thr275)
Other Name	Dual specificity mitogen-activated protein kinase kinase 7; / MAP kinase kinase 7; / MAPKK 7; / JNK-activating kinase 2; / MAPK/ERK kinase 7; / MEK 7; / Stress-activated protein kinase kinase 4; / SAPK kinase 4; / SAPKK-4; / SAPKK4; / c-Jun N-terminal kinase kinase 2; / JNK kinase 2; / JNKK 2
Species	Human
Gene ID	5609
Uniprot	O14733,
Species.1	Mouse
Gene ID.1	26400
Uniprot.1	Q8CE90,
Species.2	Rat
Gene ID.2	363855
Uniprot.2	Q4KSH7
Cellular Localization	Nucleus. Cytoplasm .
Tissue Expression	Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway
Research Areas	>>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Protein processing in endoplasmic reticulum; / >>Osteoclast differentiation; / >>Tight junction; / >>Toll-like receptor signaling pathway; / >>T cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>TNF signaling pathway; / >>Neurotrophin signaling pathway; / >>GnRH signaling pathway; / >>Relaxin signaling pathway; / >>Alcoholic liver disease; / >>Alzheimer disease; / >>Huntington disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Salmonella infection; / >>Yersinia infection; / >>Hepatitis B; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection; / >>Chemical carcinogenesis - reactive oxygen species; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation by specific MAP kinase kinase kinases such as MAP3K1/MEKK1, MAP3K3/MEKK3, MAP3K11/MLK3 and MAP3K12/DLK.,Function:Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.,PTM:Activated by phosphorylation on Ser/Thr.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.,

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MKK7 (Phospho Ser271/Thr275) rabbit pAb

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MKK7 (Phospho Ser271/Thr275) rabbit pAb

Product details

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