Price range: $268.00 through $328.00
Background:
The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Specific polymorphisms in this gene have been shown to affect the resting heart rate and can be involved in heart failure. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | AR-β1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 51kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADRB1. AA range:281-330 |
| Specificity | AR-β1 Polyclonal Antibody detects endogenous levels of AR-β1 protein. |
| Gene Name | ADRB1 |
| Protein Name | Beta-1 adrenergic receptor |
| Other Name | ADRB1; / ADRB1R; / B1AR; / Beta-1 adrenergic receptor; / Beta-1 adrenoreceptor; / Beta-1 adrenoceptor |
| Species | Human |
| Gene ID | 153 |
| Uniprot | P08588, |
| Species.1 | Mouse |
| Gene ID.1 | 11554 |
| Uniprot.1 | P34971, |
| Species.2 | Rat |
| Gene ID.2 | 24925 |
| Uniprot.2 | P18090 |
| Cellular Localization | Cell membrane; Multi-pass membrane protein . Early endosome. Colocalizes with RAPGEF2 at the plasma membrane (By similarity). Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression. . |
| Tissue Expression | Placenta, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>Calcium signaling pathway; / >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Adrenergic signaling in cardiomyocytes; / >>Gap junction; / >>Regulation of lipolysis in adipocytes; / >>Renin secretion; / >>Salivary secretion; / >>Chemical carcinogenesis - receptor activation; / >>Dilated cardiomyopathy |
| Function | Domain:The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.,Function:Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.,PTM:Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.,similarity:Belongs to the G-protein coupled receptor 1 family.,subcellular location:Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.,subunit:Interacts with GOPC, MAGI3 and DLG4., |
