Product details

Background:

catalytic activity:Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate., domain:The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+)., enzyme regulation:Stimulated by cytosolic Ca(2+)., function:Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position., similarity:Contains 1 C2 domain., similarity:Contains 1 PLA2c domain., subcellular location:Translocates to lysosomal membranes in a calcium-dependent fashion.,

Specifications

Size	50μL/100μL
Target	cPLA2-ε
Reactivity	Human, Mouse
Applications	IHC, IF, ELISA
MW(Calculated)	96kD
MW(Observed)
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human PLA2G4E. AA range:401-450
Specificity	cPLA2-ε Polyclonal Antibody detects endogenous levels of cPLA2-ε protein.
Gene Name	PLA2G4E
Protein Name	Cytosolic phospholipase A2 epsilon
Other Name	PLA2G4E; / Cytosolic phospholipase A2 epsilon; / cPLA2-epsilon; / Phospholipase A2 group IVE
Species	Human
Gene ID	123745
Uniprot	Q3MJ16,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q50L42
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm, cytosol . Early endosome membrane; Peripheral membrane protein; Cytoplasmic side . Lysosome membrane; Peripheral membrane protein; Cytoplasmic side . Cell membrane; Peripheral membrane protein; Cytoplasmic side . Targeted to clathrin-independent endocytotic vesicles through binding to phosphoinositides, especially phosphatidylinositol 4,5-bisphosphates. .
Tissue Expression	Heart,Lung,Tongue,
Signaling_pathway	Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway
Research Areas	>>Glycerophospholipid metabolism; / >>Ether lipid metabolism; / >>Arachidonic acid metabolism; / >>Linoleic acid metabolism; / >>alpha-Linolenic acid metabolism; / >>Metabolic pathways; / >>MAPK signaling pathway; / >>Ras signaling pathway; / >>Phospholipase D signaling pathway; / >>Necroptosis; / >>Vascular smooth muscle contraction; / >>VEGF signaling pathway; / >>Platelet activation; / >>Fc epsilon RI signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Glutamatergic synapse; / >>Serotonergic synapse; / >>Long-term depression; / >>Inflammatory mediator regulation of TRP channels; / >>GnRH signaling pathway; / >>Ovarian steroidogenesis; / >>Oxytocin signaling pathway; / >>Choline metabolism in cancer
Function	Catalytic activity:Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.,Domain:The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).,enzyme regulation:Stimulated by cytosolic Ca(2+).,Function:Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PLA2c domain.,subcellular location:Translocates to lysosomal membranes in a calcium-dependent fashion.,

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cPLA2-ε Polyclonal Antibody

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cPLA2-ε Polyclonal Antibody

Product details

Specifications

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