Price range: $268.00 through $328.00
Background:
This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010],
| Size | 50μL/100μL |
| Target | DNA pol λ |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 63kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DNA Polymerase lambda. AA range:451-500 |
| Specificity | DNA pol λ Polyclonal Antibody detects endogenous levels of DNA pol λ protein. |
| Gene Name | POLL |
| Protein Name | DNA polymerase lambda |
| Other Name | POLL; / DNA polymerase lambda; / Pol Lambda; / DNA polymerase beta-2; / Pol beta2; / DNA polymerase kappa |
| Species | Human |
| Gene ID | 27343 |
| Uniprot | Q9UGP5, |
| Species.1 | Mouse |
| Gene ID.1 | 56626 |
| Uniprot.1 | Q9QXE2, |
| Species.2 | Rat |
| Gene ID.2 | 361767 |
| Uniprot.2 | Q5RKI3 |
| Cellular Localization | Nucleus . Chromosome . Accumulates at sites of DNA damage. . |
| Tissue Expression | Expressed in a number of tissues. Abundant in testis. |
| Signaling_pathway | |
| Research Areas | >>Base excision repair; / >>Non-homologous end-joining |
| Function | Catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Manganese.,Function:Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the DNA polymerase type-X family.,similarity:Contains 1 BRCT domain.,subunit:Binds PCNA.,tissue specificity:Expressed in a number of tissues. Abundant in testis., |
