Price range: $268.00 through $328.00
Background:
HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | HLA-DOα |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 34kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HLA-DOA. AA range:71-120 |
| Specificity | HLA-DOα Polyclonal Antibody detects endogenous levels of HLA-DOα protein. |
| Gene Name | HLA-DOA |
| Protein Name | HLA class II histocompatibility antigen DO alpha chain |
| Other Name | HLA-DOA; / HLA-DNA; / HLA-DZA; / HLA class II histocompatibility antigen; / DO alpha chain; / MHC DN-alpha; / MHC DZ alpha; / MHC class II antigen DOA |
| Species | Human |
| Gene ID | 3111 |
| Uniprot | P06340 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Complexes with HLA-DM molecule during intracellular transport and in endosomal/lysosomal compartments. Heterotetramerization is necessary to exit the ER. |
| Tissue Expression | Lymph, |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Phagosome;Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Antigen processing and presentation;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> Intestinal immune network for IgA production;Human Diseases >> Immune disease >> Asthma;Human Diseases >> Immune disease >> Systemic lupus erythematosus;Human Diseases >> Immune disease >> Rheumatoid arthritis;Human Diseases >> Immune disease >> Autoimmune thyroid disease;Human Diseases >> Immune disease >> Inflammatory bowel disease;Human Diseases >> Immune disease >> Allograft rejection;Human Diseases >> Immune disease >> Graft-versus-host disease;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules |
| Research Areas | >>Phagosome; / >>Cell adhesion molecules; / >>Antigen processing and presentation; / >>Hematopoietic cell lineage; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>Intestinal immune network for IgA production; / >>Type I diabetes mellitus; / >>Leishmaniasis; / >>Toxoplasmosis; / >>Staphylococcus aureus infection; / >>Tuberculosis; / >>Influenza A; / >>Human T-cell leukemia virus 1 infection; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Asthma; / >>Autoimmune thyroid disease; / >>Inflammatory bowel disease; / >>Systemic lupus erythematosus; / >>Rheumatoid arthritis; / >>Allograft rejection; / >>Graft-versus-host disease; / >>Viral myocarditis |
| Function | Function:Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule.,polymorphism:The only allele of DOA known is DOA*0101 which is shown here.,similarity:Belongs to the MHC class II family.,similarity:Contains 1 Ig-like C1-type (immunoglobulin-like) domain.,subunit:Heterodimer of an alpha chain (DOA) and a beta chain (DOB)., |
