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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/SphK2 Polyclonal Antibody
SKU: WP14555 Categories: ,

SphK2 Polyclonal Antibody

Ucallm_WP14555-50

Price range: $268.00 through $328.00

SKU: WP14555-100
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Product details

Background:

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011],

Specifications

Size50μL/100μL
TargetSphK2
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)70kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human SPHK2. AA range:580-629
SpecificitySphK2 Polyclonal Antibody detects endogenous levels of SphK2 protein.
Gene NameSPHK2
Protein NameSphingosine kinase 2
Other NameSPHK2; / Sphingosine kinase 2; / SK 2; / SPK 2
SpeciesHuman
Gene ID56848
UniprotQ9NRA0,
Species.1Mouse
Gene ID.156632
Uniprot.1Q9JIA7
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm . Nucleus . Endoplasmic reticulum . Mitochondrion inner membrane . In nucleus, located in nucleosomes where it associates with core histone proteins such as histone 3 (PubMed:19729656). In brains of patients with Alzheimer's disease, may be preferentially localized in the nucleus. Cytosolic expression decrease correlates with the density of amyloid deposits (PubMed:29615132). In apoptotic cells, colocalizes with CASP1 in cell membrane where is cleaved and released from cells in an active form (PubMed:20197547). .; [Isoform 2]: Lysosome membrane .
Tissue ExpressionMainly expressed in adult kidney, liver, and brain (PubMed:10751414). Expressed in cerebral cortex and hippocampus (at protein level) (PubMed:29615132). Isoform 1 is the predominant form expressed in most tissues (PubMed:16103110).
Signaling_pathwayOrganismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway
Research Areas>>Sphingolipid metabolism; / >>Metabolic pathways; / >>Calcium signaling pathway; / >>Sphingolipid signaling pathway; / >>Phospholipase D signaling pathway; / >>VEGF signaling pathway; / >>Apelin signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Tuberculosis
FunctionAlternative products:Experimental confirmation may be lacking for some isoforms,Catalytic activity:ATP + sphinganine = ADP + sphinganine 1-phosphate.,Catalytic activity:ATP + sphingosine = ADP + sphingosine 1-phosphate.,cofactor:Magnesium.,Function:Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine.,similarity:Contains 1 DAGKc domain.,
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