Background：The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
| Item | Details |
|---|---|
| Bad Rabbit mAb | WR4119 |
| 0.22 Nitrocellulose Membrane | W3N21 |
| Item | Details |
|---|---|
| pre-staining standard protein Marker | (10-180kDa):W2100 |
| Lane Number | Cell Information |
|---|---|
| Lane1 | HL60 – Human promyelocytic leukemia cell |
| Lane2 | HUVEC – Human umbilical vein endothelial cell |
| Lane3 | Human CAF – Human cancer-associated fibroblast |
| Lane4 | HCT116 – Human colorectal carcinoma |
| Lane5 | A549 – Human lung carcinoma |
| Lane6 | Jurkat – Human T lymphocyte leukemia |
| Lane7 | HT22 – Mouse hippocampal neuronal |
| Lane8 | Thp-1 – Human monocytic leukemia |
| Lane9 | HK-2 – Human proximal tubular epithelial |
| Lane10 | PC-12 – Rat adrenal pheochromocytoma |
| Lane11 | 3T3-L1 – Mouse embryonic fibroblast cells |
| Lane12 | PC-3 – Human prostate adenocarcinoma |
| Lane13 | U-14 – Mouse cervical carcinoma |
| Lane14 | 293F – HEK293 derivative, adapted for suspension culture |
| Item | Details |
|---|---|
| SDS-PAGE separation | Various whole cell lysates were separated by 4-20% SDS-PAGE |
| Primary antibody conditions | Used at 4°C, over night with a 1:2500 dilution |
| Detection antibody | Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446) |
| Item | Band Size |
|---|---|
| Predicted band size | 18 kDa |
| Observed band size | 23 kDa |
Elevate your programmed cell death and BCL-2 family research with the Bad Rabbit Monoclonal antibody (WR4119, Bad Rabbit mAb)—a Google-optimized, rigorously validated tool for specific Bad protein detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, A549, etc.), this antibody delivers clear, consistent banding (18 kDa predicted / 23 kDa observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a robust Dylight 800-conjugated Goat anti-Rabbit detection system.
Ideal for studying Bad’s roles as a BCL-2 family member, proapoptotic regulator (forming heterodimers with BCL-xL/BCL-2 to reverse their death-repressor activity), or its phosphorylation-mediated regulation (by AKT, MAP kinase, and calcineurin)—critical for programmed cell death research, cancer apoptosis signaling, and cell survival pathway studies—this trusted Bad Rabbit Monoclonal antibody boosts assay reproducibility for apoptosis biology, oncology, and signal transduction research. Order WR4119 today to advance your Bad-focused experiments with a SEO-verified, high-performance research tool tailored for BCL-2 family apoptosis and cell survival regulation studies.
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