We present a specialized research tool designed for precise therapeutic target validation in experimental studies. The CD86 rabbit monoclonal antibody, also known as the B7-2 antibody, provides exceptional specificity for identifying costimulatory molecules on antigen-presenting cells. This includes dendritic cells, macrophages, and activated B cells. This immunological marker plays a critical role in immune response […]
We provide specialized research tools designed to investigate critical cellular mechanisms in DNA repair and cancer biology. Poly(ADP-ribose) polymerase 1 functions as a chromatin-associated enzyme that modifies nuclear proteins through poly(ADP-ribosyl)ation. This DNA-dependent process plays essential roles in cellular differentiation, proliferation, and tumor transformation. Understanding how this enzyme responds to DNA damage requires precise detection […]
Achieving reliable protein detection in Western blotting requires precise methodology and validated reagents. The PMS2 Rabbit Monoclonal Antibody serves as a critical tool for identifying DNA mismatch repair proteins in cellular lysates. We provide comprehensive Western blotting protocols that ensure reproducible results across diverse laboratory applications. This DNA mismatch repair protein plays an essential role […]
Protein targeting research demands highly specific detection tools that deliver consistent results across multiple experimental platforms. We present a comprehensive characterization of a validated immunoreagent designed for investigating membrane-cytoskeleton dynamics in cellular systems. Ezrin, also known as VIL2 or cytovillin, serves as a critical linker between cellular membranes and the cytoskeleton. This multidomain protein regulates […]
Glyceraldehyde-3-phosphate dehydrogenase serves as a critical housekeeping protein in cellular metabolism. Researchers rely on GAPDH detection for protein normalization and quality control across diverse experimental protocols. This essential enzyme maintains consistent expression levels in most cell types, making it an ideal reference marker. We recognize that rabbit monoclonal antibodies offer superior specificity compared to conventional […]
Learn how to effectively perform non-adherent cells Western blot analysis with our step-by-step guide. Master cell harvesting, lysis, and protein extraction techniques for accurate results.
Learn why Secondary Antibody in Western Blot is crucial for protein detection and visualization. Discover its role in signal amplification and successful immunoblotting experiments.
Discover the optimal Western blot electrophoresis time for your experiments. Learn factors affecting run duration and get expert tips for achieving clear protein separation bands.
Modern laboratory research demands precise methodologies for analyzing modified cellular components. We provide comprehensive guidance for detecting and characterizing these critical molecular modifications through established scientific protocols. Our approach integrates three essential analytical techniques. Western blotting offers reliable detection using advanced substrate systems and specialized gel matrices. Single molecule analysis delivers unprecedented resolution for individual […]
The question of whether to change medium before adding CCK-8 depends entirely on your experimental setup. As a matter of fact, medium replacement is essential when testing substances with oxidizing or reducing properties. This critical step prevents interference that could compromise your cell viability results. We recommend following a specific protocol when dealing with reactive […]
The viewpoint that phosphorylation fundamentally transforms cellular processes through precise molecular modifications is absolutely correct. As a matter of fact, this post-translational modification represents one of the most critical regulatory mechanisms in biological systems. This is mainly reflected in the following aspects of cellular control and signal transduction. We observe that protein phosphorylation mechanism operates […]
We recognize that accurate detection methods are essential for studying these cellular changes. Western blotting has emerged as the gold standard technique for analyzing protein modifications in laboratory settings. This method provides researchers with reliable, quantitative results that drive scientific discovery forward. The technique offers unmatched precision for identifying specific protein states within complex biological […]