CDK2 Rabbit Monoclonal Antibody

CDK2 Rabbit Monoclonal Antibody

Background:Cyclin dependent kinase 2(CDK2) Homo sapiens This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

Item Details
CDK2 Rabbit mAb WR4125
0.22 Nitrocellulose Membrane W3N21
Item Details
pre-staining standard protein Marker (10-180kDa):W2100
Lane Number Cell Information
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Item Details
SDS-PAGE separation Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary antibody conditions Used at 4°C, over night with a 1:2500 dilution
Detection antibody Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446)
Item Band Size
Predicted band size 34 kDa
Observed band size 34 kDa

Elevate your cell cycle regulation and G1-to-S phase transition research with the CDK2 Rabbit Monoclonal Antibody (WR4125)—a Google-optimized, rigorously validated tool for specific CDK2 detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, A549, etc.), this antibody delivers perfect band alignment (34 kDa predicted/observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a robust Dylight 800-conjugated Goat anti-Rabbit detection system.

Ideal for studying CDK2’s roles as a serine/threonine protein kinase in cell cycle regulation, its critical function in the G1-to-S phase transition, associations with cyclin A/E or CDK inhibitors (p21Cip1, p27Kip1) in the cyclin-dependent kinase complex, or its relevance to cell proliferation dysregulation in cancer—critical for cell cycle biology, oncology, and signal transduction research—this trusted CDK2 Rabbit Monoclonal Antibody boosts assay reproducibility for cell cycle progression studies, cancer cell proliferation research, and cyclin-kinase complex mechanism studies. Order WR4125 today to advance your CDK2-focused experiments with a SEO-verified, high-performance research tool tailored for cell cycle regulation and G1-S phase transition studies.

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