Background:Cyclin dependent kinase 2(CDK2) Homo sapiens This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.
| Item | Details |
|---|---|
| CDK2 Rabbit mAb | WR4125 |
| 0.22 Nitrocellulose Membrane | W3N21 |
| Item | Details |
|---|---|
| pre-staining standard protein Marker | (10-180kDa):W2100 |
| Lane Number | Cell Information |
|---|---|
| Lane1 | HL60 – Human promyelocytic leukemia cell |
| Lane2 | HUVEC – Human umbilical vein endothelial cell |
| Lane3 | Human CAF – Human cancer-associated fibroblast |
| Lane4 | HCT116 – Human colorectal carcinoma |
| Lane5 | A549 – Human lung carcinoma |
| Lane6 | Jurkat – Human T lymphocyte leukemia |
| Lane7 | HT22 – Mouse hippocampal neuronal |
| Lane8 | Thp-1 – Human monocytic leukemia |
| Lane9 | HK-2 – Human proximal tubular epithelial |
| Lane10 | PC-12 – Rat adrenal pheochromocytoma |
| Lane11 | 3T3-L1 – Mouse embryonic fibroblast cells |
| Lane12 | PC-3 – Human prostate adenocarcinoma |
| Lane13 | U-14 – Mouse cervical carcinoma |
| Lane14 | 293F – HEK293 derivative, adapted for suspension culture |
| Item | Details |
|---|---|
| SDS-PAGE separation | Various whole cell lysates were separated by 4-20% SDS-PAGE |
| Primary antibody conditions | Used at 4°C, over night with a 1:2500 dilution |
| Detection antibody | Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446) |
| Item | Band Size |
|---|---|
| Predicted band size | 34 kDa |
| Observed band size | 34 kDa |
Elevate your cell cycle regulation and G1-to-S phase transition research with the CDK2 Rabbit Monoclonal Antibody (WR4125)—a Google-optimized, rigorously validated tool for specific CDK2 detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, A549, etc.), this antibody delivers perfect band alignment (34 kDa predicted/observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a robust Dylight 800-conjugated Goat anti-Rabbit detection system.
Ideal for studying CDK2’s roles as a serine/threonine protein kinase in cell cycle regulation, its critical function in the G1-to-S phase transition, associations with cyclin A/E or CDK inhibitors (p21Cip1, p27Kip1) in the cyclin-dependent kinase complex, or its relevance to cell proliferation dysregulation in cancer—critical for cell cycle biology, oncology, and signal transduction research—this trusted CDK2 Rabbit Monoclonal Antibody boosts assay reproducibility for cell cycle progression studies, cancer cell proliferation research, and cyclin-kinase complex mechanism studies. Order WR4125 today to advance your CDK2-focused experiments with a SEO-verified, high-performance research tool tailored for cell cycle regulation and G1-S phase transition studies.
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