Background：Cleaved PARP1 is encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
| Category | Name/Description | Details |
|---|---|---|
| Reagent | Cleaved PARP1 Rabbit mAb | WR4004 |
| Reagent | 0.22 Nitrocellulose Membrane | W3N21 |
| Reagent | pre-staining standard protein Marker(10-250kDa) | W2101 |
| Lane 1 | Cell Type | HL60 – Human promyelocytic leukemia cell |
| Lane 2 | Cell Type | HUVEC – Human umbilical vein endothelial cell |
| Lane 3 | Cell Type | Human CAF – Human cancer-associated fibroblast |
| Lane 4 | Cell Type | HCT116 – Human colorectal carcinoma |
| Lane 5 | Cell Type | A549 – Human lung carcinoma |
| Lane 6 | Cell Type | Jurkat – Human T lymphocyte leukemia |
| Lane 7 | Cell Type | HT22 – Mouse hippocampal neuronal |
| Lane 8 | Cell Type | Thp-1 – Human monocytic leukemia |
| Lane 9 | Cell Type | HK-2 – Human proximal tubular epithelial |
| Lane 10 | Cell Type | PC-12 – Rat adrenal pheochromocytoma |
| Lane 11 | Cell Type | 3T3-L1 – Mouse embryonic fibroblast cells |
| Lane 12 | Cell Type | PC-3 – Human prostate adenocarcinoma |
| Lane 13 | Cell Type | U-14 – Mouse cervical carcinoma |
| Lane 14 | Cell Type | 293F – HEK293 derivative, adapted for suspension culture |
| Experimental Condition – Electrophoresis | SDS-PAGE | 4-20% |
| Experimental Condition – Primary Antibody | Incubation and Dilution | 4°C, over night with a 1:5000 dilution |
| Experimental Condition – Secondary Antibody | Dylight 800-conjugated Goat anti-Rabbit antibody | Cat:WS0446 |
| Band Size – Predicted | Size | 25 kDa |
| Band Size – Observed | Size | 25 kDa |
Elevate your research with Cleaved PARP1 Rabbit Recombinant Monoclonal Antibody (Cat. No.: WR4004) — a high-performance tool designed for precise PARP1 detection! As a recombinant monoclonal antibody, it delivers exceptional specificity and consistency, outperforming conventional antibodies in Western Blot applications. Validated across 14 diverse cell lines (including HL60, A549, HCT116, and primary/transformed cells from human, mouse, and rat), this antibody reliably identifies Cleaved PARP1 with a predicted and observed band size of 25 kDa — ensuring accurate, reproducible results every time. Optimized for use at a 1:5000 dilution with overnight incubation at 4°C, it pairs seamlessly with 4-20% SDS-PAGE and Dylight 800-conjugated secondary antibodies (e.g., Cat. WS0446) for sensitive detection. Whether you’re studying cancer biology, cell apoptosis, or signal transduction, Cleaved PARP1 Rabbit mAb offers unmatched reliability, backed by rigorous quality control and versatile compatibility with multiple sample types. Trust in our recombinant technology to empower your experiments — choose WR4004 for sharp, specific bands and breakthrough research outcomes!
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