Background：SQSTM1/P62 gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
| Category | Name/Description | Details |
|---|---|---|
| Reagent | SQSTM1/P62 Rabbit mAb | WR4007 |
| Reagent | 0.22 Nitrocellulose Membrane | W3N21 |
| Reagent | pre-staining standard protein Marker(10-250kDa) | W2101 |
| Lane1 | Cell Type | HL60 – Human promyelocytic leukemia cell |
| Lane2 | Cell Type | HUVEC – Human umbilical vein endothelial cell |
| Lane3 | Cell Type | Human CAF – Human cancer-associated fibroblast |
| Lane4 | Cell Type | HCT116 – Human colorectal carcinoma |
| Lane5 | Cell Type | A549 – Human lung carcinoma |
| Lane6 | Cell Type | Jurkat – Human T lymphocyte leukemia |
| Lane7 | Cell Type | HT22 – Mouse hippocampal neuronal |
| Lane8 | Cell Type | Thp-1 – Human monocytic leukemia |
| Lane9 | Cell Type | HK-2 – Human proximal tubular epithelial |
| Lane10 | Cell Type | PC-12 – Rat adrenal pheochromocytoma |
| Lane11 | Cell Type | 3T3-L1 – Mouse embryonic fibroblast cells |
| Lane12 | Cell Type | PC-3 – Human prostate adenocarcinoma |
| Lane13 | Cell Type | U-14 – Mouse cervical carcinoma |
| Lane14 | Cell Type | 293F – HEK293 derivative, adapted for suspension culture |
| Experimental Condition – Electrophoresis | SDS-PAGE | 4-20% |
| Experimental Condition – Primary Antibody | Incubation and Dilution | 4°C, over night with a 1:5000 dilution |
| Experimental Condition – Secondary Antibody | Dylight 800-conjugated Goat anti-Rabbit antibody | Cat:WS0446 |
| Band Size – Predicted | Size | 48 kDa |
| Band Size – Observed | Size | 60 kDa |
Boost your research on NF-κB signaling and Paget disease with the SQSTM1/p62 Rabbit Monoclonal Antibody (Cat. WR4007). This high-specificity reagent is engineered for precise detection, validated across 14 diverse cell lines (human, mouse, rat, including HL60, A549, HCT116, and more) via 4-20% SDS-PAGE, with a 1:5000 dilution and overnight incubation at 4°C. It targets SQSTM1/p62—a multifunctional protein central to ubiquitin binding, NF-κB pathway activation, and a key biomarker in sporadic/familial Paget disease. With a predicted band size of 48 kDa and observed 60 kDa, this antibody ensures reproducible results for Western Blot applications in cell biology, oncology, and rare disease research. Choose the SQSTM1/p62 Rabbit Monoclonal Antibody to drive accuracy and innovation in your studies of ubiquitin signaling, NF-κB regulation, and beyond.
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