FOXP3 Rabbit Monoclonal Antibody

FOXP3 Rabbit Monoclonal Antibody

Background:The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Item Details
FOXP3 Rabbit mAb WR4112
0.22 Nitrocellulose Membrane W3N21
Item Details
pre-staining standard protein Marker (10-180kDa):W2100
Lane Number Cell Information
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Item Details
SDS-PAGE separation Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary antibody conditions Used at 4°C, over night with a 1:1000 dilution
Detection antibody Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446)
Item Band Size
Predicted band size 47 kDa
Observed band size 47 kDa

Elevate your immunology and transcriptional regulation research with the FOXP3 Rabbit Monoclonal Antibody (WR4112)—a Google-optimized, rigorously validated tool for specific FOXP3 detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, Jurkat, etc.), this antibody delivers perfect band alignment (47 kDa predicted/observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:1000 dilution) and a reliable Dylight 800-conjugated Goat anti-Rabbit detection system.

Ideal for studying FOXP3’s roles as a forkhead/winged-helix family transcriptional regulator or its association with immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), this trusted FOXP3 Rabbit Monoclonal Antibody boosts assay reproducibility for autoimmunity, immunodeficiency, and immune cell biology research. Order WR4112 today to advance your FOXP3-focused experiments with a SEO-verified, high-performance research tool tailored for immunological and transcriptional regulation studies.

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