KAP-1 Rabbit Monoclonal Antibody

KAP-1 Rabbit Monoclonal Antibody

Background:The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Item Details
KAP1 Rabbit mAb WR4100
0.22 Nitrocellulose Membrane W3N21
Item Details
pre-staining standard protein Marker (10-180kDa):W2100
Lane Number Cell Information
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Item Details
SDS-PAGE separation Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary antibody conditions Used at 4°C, over night with a 1:2500 dilution
Detection antibody Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446)
Item Band Size
Predicted band size 89 kDa
Observed band size 110 kDa

Elevate your transcriptional regulation and chromatin biology research with the KAP-1 Rabbit Monoclonal Antibody (WR4100)—a Google-optimized, rigorously validated tool for specific KAP-1 detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, A549, etc.), this antibody delivers clear banding (89 kDa predicted / 110 kDa observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a robust Dylight 800-conjugated detection system.

Ideal for studying KAP-1’s roles in Kruppel-associated box domain-mediated transcriptional control, chromatin region association, or its functions as a tripartite motif family protein (featuring RING, B-box, and coiled-coil domains), this trusted KAP-1 Rabbit Monoclonal Antibody boosts assay reproducibility. Order WR4100 today to advance your KAP-1-focused experiments with a SEO-verified, high-performance research tool tailored for transcriptional and chromatin biology studies.

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