Specifications
| Size | 50μL/100μL |
| Target | 14-3-3 eta |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| MW(Calculated) | |
| MW(Observed) | 28kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Monoclonal |
| Clone Number | 5B2 |
| Immunogen | Synthesized peptide derived from human 14-3-3 eta |
| Specificity | This antibody detects endogenous levels of 14-3-3 eta at Human, Mouse,Rat |
| Gene Name | YWHAH YWHA1 |
| Protein Name | 14-3-3 eta |
| Other Name | YWHAH; / YWHA1; / 14-3-3 protein eta; / Protein AS1 |
| Species | Human |
| Gene ID | 7533 |
| Uniprot | Q04917, |
| Species.1 | Mouse |
| Gene ID.1 | 22629 |
| Uniprot.1 | P68510, |
| Species.2 | Rat |
| Gene ID.2 | 25576 |
| Uniprot.2 | P68511 |
| Cellular Localization | |
| Tissue Expression | Expressed mainly in the brain and present in other tissues albeit at lower levels. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | |
| Function | Function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,similarity:Belongs to the 14-3-3 family.,subunit:Homodimer (By similarity). Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with RGNEF and PCTK1 (By similarity). Weakly interacts with CDKN1B.,tissue specificity:Expressed mainly in the brain and present in other tissues albeit at lower levels., |
