SKU: WP10864 Categories: ,

9G8 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP10864-100
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Product details

Background:

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010],

Specifications

Size50μL/100μL
Target9G8
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)35kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human SFRS7. AA range:61-110
Specificity9G8 Polyclonal Antibody detects endogenous levels of 9G8 protein.
Gene NameSRSF7
Protein NameSerine/arginine-rich splicing factor 7
Other NameSRSF7; / SFRS7; / Serine/arginine-rich splicing factor 7; / Splicing factor 9G8; / Splicing factor; / arginine/serine-rich 7
SpeciesHuman
Gene ID6432
UniprotQ16629,
Species.1Mouse
Gene ID.1225027
Uniprot.1Q8BL97
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus . Cytoplasm .
Tissue ExpressionBrain, liver, kidney and lung.
Signaling_pathwayHuman Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis
Research Areas>>Spliceosome; / >>Amyotrophic lateral sclerosis; / >>Herpes simplex virus 1 infection
FunctionAlternative products:Isoforms, often lacking the RS domain and differentially expressed in fetal tissues, may be involved in modulation of 9G8 function,Function:Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro.,PTM:Extensively phosphorylated on serine residues in the RS domain.,similarity:Belongs to the splicing factor SR family.,similarity:Contains 1 CCHC-type zinc finger.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Found in large molecular weight complexes containing CCNL1 and the p110 isoforms of either CDC2L1 or CDC2L2. Interacts with CCNL2 and CPSF6.,tissue specificity:Brain, liver, kidney and lung.,
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