Price range: $268.00 through $328.00
Background:
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012],
| Size | 50μL/100μL |
| Target | A-Raf |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 68kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:20000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human A-RAF. AA range:241-290 |
| Specificity | A-Raf Polyclonal Antibody detects endogenous levels of A-Raf protein. |
| Gene Name | ARAF |
| Protein Name | Serine/threonine-protein kinase A-Raf |
| Other Name | ARAF; / ARAF1; / PKS; / PKS2; / Serine/threonine-protein kinase A-Raf; / Proto-oncogene A-Raf; / Proto-oncogene A-Raf-1; / Proto-oncogene Pks |
| Species | Human |
| Gene ID | 369 |
| Uniprot | P10398, |
| Species.1 | Mouse |
| Gene ID.1 | 11836 |
| Uniprot.1 | P04627, |
| Species.2 | Rat |
| Gene ID.2 | 64363 |
| Uniprot.2 | P14056 |
| Cellular Localization | intracellular,mitochondrion,cytosol, |
| Tissue Expression | Predominantly in urogenital tissues. |
| Signaling_pathway | Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Cancer: specific types >> Bladder cancer;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway |
| Research Areas | >>EGFR tyrosine kinase inhibitor resistance; / >>Endocrine resistance; / >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>FoxO signaling pathway; / >>Vascular smooth muscle contraction; / >>Natural killer cell mediated cytotoxicity; / >>Long-term potentiation; / >>Serotonergic synapse; / >>Long-term depression; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>Progesterone-mediated oocyte maturation; / >>Parathyroid hormone synthesis, secretion and action; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Alcoholism; / >>Hepatitis C; / >>Hepatitis B; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Chemical carcinogenesis - reactive oxygen species; / >>Colorectal cancer; / >>Renal cell carcinoma; / >>Pancreatic cancer; / >>Endometrial cancer; / >>Glioma; / >>Prostate cancer; / >>Melanoma; / >>Bladder cancer; / >>Chronic myeloid leukemia; / >>Acute myeloid leukemia; / >>Non-small cell lung cancer; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2 zinc ions per subunit.,Function:Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RBD (Ras-binding) domain.,subunit:Interacts with TH1L/NELFD.,tissue specificity:Predominantly in urogenital tissues., |
