Price range: $268.00 through $328.00
Background:
ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008],
| Size | 50μL/100μL |
| Target | ABCB5 |
| Reactivity | Human |
| Applications | WB, IHC, IF |
| MW(Calculated) | |
| MW(Observed) | 90kD |
| Host Species | Mouse |
| Isotype | IgG1,kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000; IF 1:200; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 11A2 |
| Immunogen | Synthetic Peptide of ABCB5 AA range: 130-180 |
| Specificity | The antibody detects endogenous ABCB5 proteins. |
| Gene Name | ABCB5 |
| Protein Name | ATP-binding cassette sub-family B member 5 |
| Other Name | ABCB5; / ATP-binding cassette sub-family B member 5; / ABCB5 P-gp; / P-glycoprotein ABCB5 |
| Species | Human |
| Gene ID | 340273 |
| Uniprot | Q2M3G0 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Multi-pass membrane protein . |
| Tissue Expression | Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Highly expressed in several malignant tissues: highly expressed in clinical melanomas, with low expression in normal skin. In melanoma, marks malignant melanoma-initiating cells (MMIC), in which clinical virulence resides as a consequence of unlimited self-renewal capacity, resulting in inexorable tumor progression and metastasis. Also highly expressed in a number of leukemia cells. Expressed in basal limbal epithelium. |
| Signaling_pathway | |
| Research Areas | >>ABC transporters |
| Function | Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,Caution:Was named ABCB1 by some authors.,Function:Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas.,miscellaneous:Depletion of ABCB5 by RNAi increases the sensitivity to several drugs of a subset of melanoma cells.,similarity:Belongs to the ABC transporter family.,similarity:Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.,similarity:Contains 1 ABC transmembrane type-1 domain.,similarity:Contains 1 RRM (RNA recognition motif) domain.,similarity:Contains 2 ABC transporter domains.,subunit:Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.,tissue specificity:Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues.,tissue specificity:Expressed in heart, liver, skeletal muscle and pancreas., |
