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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/ABHD4 Polyclonal Antibody
SKU: WP10896 Categories: ,

ABHD4 Polyclonal Antibody

Ucallm_WP10896-50

Price range: $268.00 through $328.00

SKU: WP10896-100
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Product details

Background:

caution:Thr-291 is present instead of the conserved His which is expected to be an active site residue., function:Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine., similarity:Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.,

Specifications

TargetABHD4
ReactivityHuman, Mouse
ApplicationsIHC, IF, ELISA
MW(Calculated)39kD
MW(Observed)
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Modification
Recommended Dilution RatioIHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human ABHD4. AA range:251-300
Sequence
SpecificityABHD4 Polyclonal Antibody detects endogenous levels of ABHD4 protein.
Gene NameABHD4
Protein NameAbhydrolase domain-containing protein 4
Other NameABHD4; / Abhydrolase domain-containing protein 4; / Alpha/beta-hydrolase 4; / Lyso-N-acylphosphatidylethanolamine lipase
SpeciesHuman
Gene ID-163874
UniprotQ8TB40,
Species.1Mouse
Gene ID-2105501
Uniprot.1Q8VD66
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Backgroundcaution:Thr-291 is present instead of the conserved His which is expected to be an active site residue.,function:Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.,similarity:Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.,
Cellular Localizationendoplasmic reticulum membrane,lipid particle,
Tissue ExpressionCervix,
Signaling_pathway
Research Areas
FunctionCaution:Thr-291 is present instead of the conserved His which is expected to be an active site residue.,Function:Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.,similarity:Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.,
RRID
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