SKU: WP0268 Categories: ,

Acetyl eIF5A/eIF5A2 (K47) Polyclonal Antibody

Price range: $288.00 through $368.00

SKU: WP0268-100
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Product details

Background:

function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond., PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine)., similarity:Belongs to the eIF-5A family., tissue specificity:Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.,

Specifications

TargeteIF5A2
ReactivityHuman, Mouse, Rat
ApplicationsWB, ELISA
MW(Calculated)17kD
MW(Observed)
Host SpeciesRabbit
IsotypeIgG
Conjugate
ModificationAcetyl
Recommended Dilution RatioWB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.
CompositionLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification ProcessThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage Condition-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized Acetyl-peptide derived from human eIF5A around the Acetylation site of Lys47. AA range:11-60
Sequence
SpecificityAcetyl-eIF5A/eIF5A2 (K47) Polyclonal Antibody detects endogenous levels of eIF5A/eIF5A2 protein only when acetylated at K47.
Gene NameEIF5A2
Protein NameEukaryotic translation initiation factor 5A-2
AliasEIF5A2;Eukaryotic translation initiation factor 5A-2;eIF-5A-2;eIF-5A2;Eukaryotic initiation factor 5A isoform 2
Organism-1Human
Gene ID-156648
SwissProt-1Q9GZV4
Organism-2Mouse
Gene ID-2208691
SwissProt-2Q8BGY2
Organism-3
Gene ID-3
SwissProt-3
Organism-4
Gene ID-4
SwissProt-4
Cellular LocalizationCytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Nucleus, nuclear pore complex . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. .
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