U-Blot® ACSL4 Rabbit mAb
WR4261 Western blot analysis of lysates from Hela cells, using ACSL4 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]
Specifications
| Size | 50μL/100μL |
| Target | ACSL4 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 78kD |
| MW(Observed) | 78kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:2000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0448R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ACSL4 ACS4 FACL4 LACS4 |
| Protein Name | ACSL4 |
| Other Name | Long-chain-fatty-acid--CoA ligase 4; / Long-chain acyl-CoA synthetase 4; / LACS 4 |
| Species | Human |
| Gene ID | 2182 |
| Uniprot | O60488, |
| Species.1 | Mouse |
| Gene ID.1 | 50790 |
| Uniprot.1 | Q9QUJ7, |
| Species.2 | Rat |
| Gene ID.2 | 113976 |
| Uniprot.2 | O35547 |
| Cellular Localization | Membrane |
| Tissue Expression | |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Peroxisome;Cellular Processes >> Cell growth and death >> Ferroptosis;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Endocrine system >> PPAR signaling pathway |
| Research Areas | >>Fatty acid biosynthesis; / >>Fatty acid degradation; / >>Metabolic pathways; / >>Fatty acid metabolism; / >>PPAR signaling pathway; / >>Peroxisome; / >>Ferroptosis; / >>Thermogenesis; / >>Adipocytokine signaling pathway |
| Function |
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