Price range: $268.00 through $328.00
Background:
adenosine deaminase, RNA specific(ADAR) Homo sapiens This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010],
| Size | 50μL/100μL |
| Target | ADAR1 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 135kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADAR1. AA range:1172-1221 |
| Specificity | ADAR1 Polyclonal Antibody detects endogenous levels of ADAR1 protein. |
| Gene Name | ADAR |
| Protein Name | Double-stranded RNA-specific adenosine deaminase |
| Other Name | ADAR; / ADAR1; / DSRAD; / G1P1; / IFI4; / Double-stranded RNA-specific adenosine deaminase; / DRADA; / 136 kDa double-stranded RNA-binding protein; / p136; / Interferon-inducible protein 4; / IFI-4; / K88DSRBP |
| Species | Human |
| Gene ID | 103 |
| Uniprot | P55265, |
| Species.1 | Mouse |
| Gene ID.1 | 56417 |
| Uniprot.1 | Q99MU3, |
| Species.2 | Rat |
| Gene ID.2 | 81635 |
| Uniprot.2 | P55266 |
| Cellular Localization | [Isoform 1]: Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus (PubMed:7565688, PubMed:24753571). Nuclear import is mediated by TNPO1 (PubMed:24753571). .; [Isoform 5]: Cytoplasm . Nucleus . Nucleus, nucleolus . Predominantly nuclear but can shuttle between nucleus and cytoplasm. TNPO1 can mediate its nuclear import whereas XPO5 can mediate its nuclear export. . |
| Tissue Expression | Ubiquitously expressed, highest levels were found in brain and lung (PubMed:7972084). Isoform 5 is expressed at higher levels in astrocytomas as compared to normal brain tissue and expression increases strikingly with the severity of the tumor, being higher in the most aggressive tumors. |
| Signaling_pathway | Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway |
| Research Areas | >>Cytosolic DNA-sensing pathway; / >>Measles; / >>Influenza A; / >>Coronavirus disease - COVID-19 |
| Function | Caution:The N-terminus of isoform 4 has been derived from EST and genomic sequences.,Disease:Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.,Function:Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.,induction:Isoform 1 is induced by interferon alpha. Isoform 5 is constitutively expressed.,PTM:Sumoylation reduces RNA-editing activity.,similarity:Contains 1 A to I editase domain.,similarity:Contains 2 DRADA repeats.,similarity:Contains 3 DRBM (double-stranded RNA-binding) domains.,subcellular location:Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus.,subunit:Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90.,tissue specificity:Ubiquitously expressed, highest levels were found in brain and lung., |
