AF-4 Polyclonal Antibody
Ucallm_WP10972-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in mu
Specifications
| Size | 50μL/100μL |
| Target | AF-4 |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 130kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AF4. AA range:1-50 |
| Specificity | AF-4 Polyclonal Antibody detects endogenous levels of AF-4 protein. |
| Gene Name | AFF1 |
| Protein Name | AF4/FMR2 family member 1 |
| Other Name | AFF1; / AF4; / FEL; / MLLT2; / PBM1; / AF4/FMR2 family member 1; / ALL1-fused gene from chromosome 4 protein; / Protein AF-4; / Protein FEL; / Proto-oncogene AF4 |
| Species | Human |
| Gene ID | 4299 |
| Uniprot | P51825, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O88573 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Epithelium,PCR rescued clones,Placenta, |
| Signaling_pathway | Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer |
| Research Areas | >>Transcriptional misregulation in cancer |
| Function | Disease:A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4; 11)(q21; q23) with MLL/HRX. The result is a rogue activator protein.,similarity:Belongs to the AF4 family., |
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