Name: Ah Receptor Polyclonal Antibody
SKU: WP10977
Price: 268 USD
Availability: InStock

Product details

Background:

The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015],

Specifications

Size	50μL/100μL
Target	AhR
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	96kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human AhR. AA range:2-51
Specificity	Ah Receptor Polyclonal Antibody detects endogenous levels of Ah Receptor protein.
Gene Name	AHR BHLHE76
Protein Name	Aryl hydrocarbon receptor
Other Name	AHR; / BHLHE76; / Aryl hydrocarbon receptor; / Ah receptor; / AhR; / Class E basic helix-loop-helix protein 76; / bHLHe76; / AHRR; / BHLHE77; / KIAA1234; / Aryl hydrocarbon receptor repressor; / AhR repressor; / AhRR; / Class E basic helix-loop-helix protein 77; / bHL
Species	Human
Gene ID	196
Uniprot	P35869,
Species.1	Mouse
Gene ID.1	11622
Uniprot.1	P30561,
Species.2	Rat
Gene ID.2	25690
Uniprot.2	P41738
Cellular Localization	Cytoplasm . Nucleus . Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus. .
Tissue Expression	Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle. Expressed in retinal photoreceptors (PubMed:29726989).
Signaling_pathway	Organismal Systems >> Immune system >> Th17 cell differentiation
Research Areas	>>Th17 cell differentiation; / >>Cushing syndrome; / >>Chemical carcinogenesis - receptor activation; / >>Chemical carcinogenesis - reactive oxygen species
Function	Function:Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.,induction:Induced or repressed by TGF-beta and dioxin in a cell-type specific fashion. Repressed by cAMP, retinoic acid, and TPA.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 PAC (PAS-associated C-terminal) domain.,similarity:Contains 2 PAS (PER-ARNT-SIM) domains.,subcellular location:Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.,subunit:Binds MYBBP1A (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. In the nucleus, heterodimer of AHR and ARNT. Interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. Interacts with NEDD8 and IVNS1ABP.,tissue specificity:Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.,