AIP4 Polyclonal Antibody
Ucallm_WP10984-50
Price range: $268.00 through $328.00
Product details
Background:
itchy E3 ubiquitin protein ligase(ITCH) Homo sapiens This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],
Specifications
| Size | 50μL/100μL |
| Target | AIP4 |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 103kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ITCH. AA range:386-435 |
| Specificity | AIP4 Polyclonal Antibody detects endogenous levels of AIP4 protein. |
| Gene Name | ITCH |
| Protein Name | E3 ubiquitin-protein ligase Itchy homolog |
| Other Name | ITCH; / E3 ubiquitin-protein ligase Itchy homolog; / Itch; / Atrophin-1-interacting protein 4; / AIP4; / NFE2-associated polypeptide 1; / NAPP1 |
| Species | Human |
| Gene ID | 83737 |
| Uniprot | Q96J02, |
| Species.1 | Mouse |
| Gene ID.1 | 16396 |
| Uniprot.1 | Q8C863 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasm . Nucleus . Early endosome membrane; Peripheral membrane protein; Cytoplasmic side . Endosome membrane; Peripheral membrane protein; Cytoplasmic side . May be recruited to exosomes by NDFIP1 (PubMed:18819914). Localizes to plasma membrane upon CXCL12 stimulation where it co-localizes with CXCL4 (PubMed:14602072). Localization to early endosomes is increased upon CXCL12 stimulation where it co-localizes with DTX3L and CXCL4 (PubMed:24790097). . |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Notch signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis |
| Research Areas | >>Ubiquitin mediated proteolysis; / >>Endocytosis; / >>TNF signaling pathway; / >>Non-alcoholic fatty liver disease |
| Function | Function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 C2 domain.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 4 WW domains.,subunit:Interacts via its WW domains with DRPLA, NFE2 and CBLC. Interacts with Epstein-Barr virus LMP2A. Interacts with NOTCH1, OCLN, JUN and JUNB. Interacts with NDFIP1 in vitro (By similarity). Interacts with ARHGEF7.,tissue specificity:Widely expressed., |
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