Price range: $268.00 through $328.00
Background:
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008],
| Size | 50μL/100μL |
| Target | ALKB3 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 31kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 110-190 |
| Specificity | ALKB3 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | ALKBH3 ABH3 DEPC1 |
| Protein Name | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (Alkylated DNA repair protein alkB homolog 3) (DEPC-1) (Prostate cancer antigen 1) |
| Other Name | |
| Species | Human |
| Gene ID | 221120 |
| Uniprot | Q96Q83, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8K1E6, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q5XIC8 |
| Cellular Localization | Nucleus . Cytoplasm . Colocalizes with ASCC2 and ASCC3 in nuclear foci when cells have been exposed to alkylating agents that cause DNA damage (PubMed:29144457). Predominantly localizes to the nucleus. . |
| Tissue Expression | Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon. |
| Signaling_pathway | |
| Research Areas | |
| Function | cofactor:Binds 1 Fe(2+) ion per subunit.,enzyme regulation:Activated by ascorbate.,Function:Dioxygenase that repairs alkylated DNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has a strong preference for single-stranded DNA. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.,similarity:Belongs to the alkB family.,tissue specificity:Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon., |
