Price range: $268.00 through $328.00
Background:The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008],
| Target | ALKBH2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, FC |
| MW(Calculated) | 29kD |
| MW(Observed) | 29kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:1000-1:5000; FC 1:100-1:500; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Source | |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | PT1116R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Sequence | |
| Specificity | Endogenous |
| Gene Name | ALKBH2 ABH2 |
| Protein Name | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 (Alkylated DNA repair protein alkB homolog 2) (DNA oxidative demethylase ALKBH2) (Oxy DC1) |
| Other Name | |
| Species | Human |
| Gene ID-1 | 121642 |
| Uniprot | Q6NS38, |
| Species.1 | Mouse |
| Gene ID-2 | |
| Uniprot.1 | Q6P6J4 |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008], |
| Cellular Localization | Nucleus . Nucleus, nucleolus . Nucleus, nucleoplasm . Relocates to the replication foci during S-phase. . |
| Tissue Expression | Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder. |
| Signaling_pathway | |
| Research Areas | |
| Function | cofactor:Binds 1 Fe(2+) ion per subunit.,enzyme regulation:Activated by ascorbate.,Function:Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.,similarity:Belongs to the alkB family.,subcellular location:Detected in replication foci during S-phase.,tissue specificity:Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder., |
| RRID |
