U-Blot® ALPP Rabbit mAb
WR4291 Western blot analysis of lysates from Hela cells, using ALPP Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015],
Specifications
| Size | 50μL/100μL |
| Target | PLAP |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 58kD |
| MW(Observed) | 70kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0484R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ALPP; PLAP |
| Protein Name | Alkaline phosphatase, placental type |
| Other Name | ALPP; / PLAP; / Alkaline phosphatase; / placental type; / Alkaline phosphatase Regan isozyme; / Placental alkaline phosphatase 1; / PLAP-1 |
| Species | Human |
| Gene ID | 250 |
| Uniprot | P05187 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Membrane |
| Tissue Expression | Detected in placenta (at protein level). |
| Signaling_pathway | |
| Research Areas | >>Thiamine metabolism; / >>Folate biosynthesis; / >>Metabolic pathways; / >>Biosynthesis of cofactors |
| Function | Catalytic activity:A phosphate monoester + H(2)O = an alcohol + phosphate.,cofactor:Binds 1 magnesium ion.,cofactor:Binds 2 zinc ions.,miscellaneous:In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).,online information:Alkaline phosphatase entry,polymorphism:Placental ALP is highly polymorphic, there are at least three common alleles.,similarity:Belongs to the alkaline phosphatase family.,subunit:Homodimer., |
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