Price range: $268.00 through $328.00
Background:
This gene encodes a flavoprotein oxidoreductase that binds single stranded DNA and is thought to contribute to apoptosis in the presence of bacterial and viral DNA. The expression of this gene is also found to be induced by tumor suppressor protein p53 in colon cancer cells. [provided by RefSeq, Nov 2010],
| Size | 50μL/100μL |
| Target | AMID |
| Reactivity | Human, Mouse, Monkey |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 41kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AIFM2. AA range:141-190 |
| Specificity | AMID Polyclonal Antibody detects endogenous levels of AMID protein. |
| Gene Name | AIFM2 |
| Protein Name | Apoptosis-inducing factor 2 |
| Other Name | AIFM2; / AMID; / PRG3; / Apoptosis-inducing factor 2; / Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; / Apoptosis-inducing factor-like mitochondrion-associated inducer of death; / p53-responsive gene 3 protein,FSP1 |
| Species | Human |
| Gene ID | 84883 |
| Uniprot | Q9BRQ8, |
| Species.1 | Mouse |
| Gene ID.1 | 71361 |
| Uniprot.1 | Q8BUE4 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Lipid droplet . Cell membrane; Lipid-anchor . Cytoplasm . Mitochondrion membrane . Nucleus . |
| Tissue Expression | Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expressed following p53/TP53 induction. The shorter 1.8 kb transcript seems to be the major transcript in EB1 colon cancer cells. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> p53 signaling pathway |
| Research Areas | >>p53 signaling pathway |
| Function | cofactor:FAD. Binds 6-hydroxy-FAD non-covalently.,Function:Oxidoreductase, which may play a role in mediating a TP53/p53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Binds to DNA in a sequence-independent manner. May contribute to genotoxin-induced growth arrest.,induction:Expression detected at 4 hours after induction by TP53/p53. Down-regulated in a wide range of human tumors.,similarity:Belongs to the FAD-dependent oxidoreductase family.,tissue specificity:Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expressed following TP53/p53 induction. The shorter 1.8 kb transcript seems to be the major transcript in EB1 colon cancer cells., |
