Name: Amphiphysin II Polyclonal Antibody
SKU: WP15379
Price: 268 USD
Availability: InStock

Product details

Background:

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq, Mar 2016],

Specifications

Size	50μL/100μL
Target	Amphiphysin II
Reactivity	Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	64kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthesized peptide derived from the C-terminal region of human Amphiphysin II.
Specificity	Amphiphysin II Polyclonal Antibody detects endogenous levels of Amphiphysin II protein.
Gene Name	BIN1
Protein Name	Myc box-dependent-interacting protein 1
Other Name	BIN1; / AMPHL; / Myc box-dependent-interacting protein 1; / Amphiphysin II; / Amphiphysin-like protein; / Box-dependent myc-interacting protein 1; / Bridging integrator 1
Species	Human
Gene ID	274
Uniprot	O00499,
Species.1	Mouse
Gene ID.1	30948
Uniprot.1	O08539,
Species.2	Rat
Gene ID.2	117028
Uniprot.2	O08839
Cellular Localization	[Isoform BIN1]: Nucleus . Cytoplasm . Endosome . Cell membrane, sarcolemma, T-tubule .; [Isoform IIA]: Cytoplasm .
Tissue Expression	Ubiquitous. Highest expression in the brain and muscle (PubMed:9182667). Expressed in oligodendrocytes (PubMed:27488240). Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter (PubMed:27488240). Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
Signaling_pathway	Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis
Research Areas	>>Endocytosis; / >>Fc gamma R-mediated phagocytosis
Function	Alternative products:Additional isoforms seem to exist,Disease:Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.,Function:May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.,PTM:Phosphorylated by protein kinase C.,similarity:Contains 1 BAR domain.,similarity:Contains 1 SH3 domain.,subunit:Heterodimer with AMPH. Binds SH3GLB1 (By similarity). Binds to SYNJ1 and DNM1 through its SH3 domain, and to clathrin through a region outside of the SH3 domain. Also binds AP2A2. Interacts with the N-terminal transactivation domain of MYC in a manner requiring the integrity of the conserved MYC box regions 1 and 2. Interacts with BIN2. Interacts with HCV NS5A through its SH3 domain.,tissue specificity:Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.,