Name: AMPKα2 Polyclonal Antibody
SKU: WP5922
Price: 268 USD
Availability: InStock

Product details

Background:

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	AMPKα2
Reactivity	Human, Mouse, Rat
Applications	WB
MW(Calculated)
MW(Observed)	62kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000-2000
Formulation	PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Polyclonal
Clone Number
Immunogen	Recombinant Protein of AMPKα2
Specificity	The antibody detects endogenous AMPKα2 protein.
Gene Name	PRKAA2
Protein Name	5'-AMP-activated protein kinase catalytic subunit alpha-2
Other Name	PRKAA2; / AMPK; / AMPK2; / 5'-AMP-activated protein kinase catalytic subunit alpha-2; / AMPK subunit alpha-2; / Acetyl-CoA carboxylase kinase; / ACACA kinase; / Hydroxymethylglutaryl-CoA reductase kinase; / HMGCR kinase
Species	Human
Gene ID	5563
Uniprot	P54646,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q8BRK8,
Species.2	Rat
Gene ID.2
Uniprot.2	Q09137
Cellular Localization	Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. .
Tissue Expression	Heart,Skeletal muscle,
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Organismal Systems >> Environmental adaptation >> Circadian rhythm;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>FoxO signaling pathway; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Apelin signaling pathway; / >>Tight junction; / >>Circadian rhythm; / >>Thermogenesis; / >>Insulin signaling pathway; / >>Adipocytokine signaling pathway; / >>Oxytocin signaling pathway; / >>Glucagon signaling pathway; / >>Insulin resistance; / >>Non-alcoholic fatty liver disease; / >>Alcoholic liver disease; / >>Hypertrophic cardiomyopathy; / >>Fluid shear stress and atherosclerosis
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,Function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Heterotrimer of a catalytic subunit, a beta and a gamma non-catalytic subunits.,

Please login to post questions

AMPKα2 Polyclonal Antibody

Product details

Specifications

About Us

Support

Order

Menu

AMPKα2 Polyclonal Antibody

Product details

Specifications

Related products

U-Blot® Smad2 (Phospho Ser250) Rabbit mAb

U-Blot® CDC37 (Phospho Ser13) Rabbit mAb

U-Blot® Cyclin B1 (Phospho Ser126) Rabbit mAb

U-Blot® Glutamine Synthetase Rabbit mAb

U-Blot® CDK6 Rabbit mAb

U-Blot® GAPDH Rabbit mAb

Customer Reviews

Menu