Name: U-Blot® AMPK α1/2 (Phospho-Thr183/Thr172) Rabbit mAb
SKU: WR4640
Price: 288 USD
Availability: InStock

U-Blot® AMPK α1/2 (Phospho-Thr183/Thr172) Rabbit mAb

Western Blot analysis using C6 was treated oligomycin(0.5μM) for 30 minutes lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour.

WR4640 Western Blot analysis using C6 was treated oligomycin(0.5μM) for 30 minutes lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour.

Price range: $288.00 through $368.00

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SKU: WR4640-50

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Product details

Background:The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

Specifications

Target	AMPKα1/2
Reactivity	Human,Mouse,Rat
Applications	WB,IF,ELISA
MW(Calculated)	62kD,64kD
MW(Observed)	62kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Phospho
Modification Site	Thr183/Thr172
Recommended Dilution Ratio	WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Protein A
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Clonality	Monoclonal
Clone Number	PT0920R
Specificity	AMPK α1/2 (Phospho-Thr183/Thr172) Antibody detects endogenous levels of Tau protein only when phosphorylated at Thr183/Thr172.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):LRtSC
Gene Name	AAPK1/AAPK2
Protein Name	5'-AMP-activated protein kinase catalytic subunit alpha-1/2
Other Name	PRKAA1;AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;Acetyl-CoA carboxylase kinase;ACACA kinase;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;Tau-protein kinase PRKAA1;PRKAA2;AMPK;
Organism-1	Human
Gene ID-1	5562;5563
SwissProt-1	Q13131;P54646
Organism-2	Mouse
Gene ID-2	105787;108079
SwissProt-2
Organism-3	Rat
Gene ID-3	65248;78975
SwissProt-3	P54645;Q09137
Organism-4
GeneID-4
SwissProt-4
Cellular Localization	Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. .
Unnamed: 33