SKU: WP15244 Categories: ,

Amylase Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP15244-100
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Product details

Background:

Amylases are secreted proteins that hydrolyze 1, 4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetAmylase
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)60kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from the N-terminal region of human AMY1/2. AA range:61-110
SpecificityAmylase Polyclonal Antibody detects endogenous levels of Amylase protein.
Gene NameAMY1A/AMY1B/AMY1C/AMY2A/AMY2B
Protein NameAlpha-amylase 1/Pancreatic alpha-amylase/Alpha-amylase 2B
Other NameAMY1A; / AMY1; / AMY1B; / AMY1C; / Alpha-amylase 1; / 1,4-alpha-D-glucan glucanohydrolase 1; / Salivary alpha-amylase; / AMY2A; / Pancreatic alpha-amylase; / PA; / 1,4-alpha-D-glucan glucanohydrolase; / AMY2B; / Alpha-amylase 2B; / 1,4-alpha-D-glucan glucanohydrolase 2B; / Carcinoid alpha-amylase
SpeciesHuman
Gene ID276;277;278;279;280
UniprotP0DUB6;P0DTE8;P0DTE7;P04746;P19961,
Species.1Mouse
Gene ID.111722
Uniprot.1P00687,
Species.2Rat
Gene ID.2497039
Uniprot.2P00689
Cellular Localizationextracellular space,extracellular exosome,
Tissue ExpressionGlandular pool- thyroid,Saliva,Thyroid,
Signaling_pathway
Research Areas
FunctionCatalytic activity:Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.,cofactor:Binds 1 calcium ion per subunit.,cofactor:Binds 1 chloride ion per subunit.,online information:Amylase entry,similarity:Belongs to the glycosyl hydrolase 13 family.,subunit:Monomer.,
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